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KMID : 0380020030180060511
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 6 p.511 ~ p.516
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Isolation and Identification of Lignans as Antioxidant from Leaves of Catalpa ovata G. Don
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Kuk Ju-Hee
Ma Seung-Jin
Moon Jae-Hak
Park Keun-Hyung
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Abstract
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The methanol extract from leaves of Catalpa ovata 6. DoN showed DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity, and its antioxidative compounds were studied. The ethyl acetate-soluble neutral fraction from the methanol extract was successively purified with silica gel adsorption column chromatography, Sephadex LH-20 column chromatography and HPLC. Three antioxidative compounds were isolated and identified as piperitol, pinoresinol and lariciresinol by HR-MS and NMR spectroscopic analyses. The DPPH radical-scavenging activity of the identified compounds decreased in the order of lariciresinol > pinoresinol > piperitol.
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KEYWORD
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Catalpa ovata, antioxidative compound, lariciresinol, pinoresinol, piperitol
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